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Recent research papers by the investigators who initiated DiscoveryLab are listed below, and represent a fraction of the research into novel targets and leads at universities in Alberta.


Patel, TR, Winzor, DJ and Scott, DJ. Allowance for radial dilution in evaluating the concentration dependence of sedimentation coefficients for globular proteins. European Biophysics Journal. in press.

Hyde EI, Callow P, Rajesekar KV, Timmins P, Patel,TR, Siligardi G, Hussain R, White SA, Thomas CM, Scott DJ. Intrinsic disorder in the partitioning protein KorB persists after cooperative complex formation with operator DNA and KorA. Biochem J 474, 3121–3135 2.

Guix FX, R Sannerud, F Berditchevski, A Arranz, K Horré, A Snellinx, A Thathiah, R Sundaresan, M Overduin, S Kumar-Singh, E Radaelli, W Annaert, B De Strooper, Tetraspanin 6: a pivotal protein of the Multiple Vesicular Body determining exosome release and lysosomal degradation of Amyloid Precursor protein fragments. Mol Neurodeger 12(1) 25.

Patel TR, Chojnowski GA, Koul A, McKenna SA, Bujnicki JM, Structural Studies of RNA-protein Complexes: A Hybrid Approach Involving Hydrodynamics, Scattering, and Computational Methods. J. M. Methods 118-119, 146-162.

Krahn N, Spearman M, Meier M, Spearman M, Dorion-Thibaudeau J, McDougall M, Patel, TR, De Crescenzo G, Durocher Y, Stetefeld J, Butler M. Inhibition of glycosylation on a camelid antibody uniquely affects its FcγRI binding activity. Eur J Pharm Sci 96, 428-439


Reuten R, Patel TR, McDougall M, Rama N, Nikodemus D, Gibert B, Delcros J, Prein C, Meier M, Metzger S, Zhou Z, Kaltenberg J, McKee KK, Bald T, Tüting T, Zigrino P, Djonov V, Bloch W, Clausen-Schaumann H, Poschl E, Yurchenco PD, Ehrba M, Mehlen P, Stetefeld J, Koch M. Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes. Nature Commun 7, 13515, 1-17

Diviani D, Raimondi F, Del Vescovo CD, Dreyer E, Reggi E, Osman H, Ruggieri L, Gonano C, Cavin S, Box CL, Lenoir M, Overduin M, Bellucci L, Seeber M, Fanelli F. Small-Molecule Protein-Protein Interaction Inhibitor of Oncogenic Rho Signaling. Cell Chem Biol. (16)30245-8.

Gräßel L, Fast LA, Scheffer KD, Boukhallouk F, Spoden GA, Tenzer S, Boller K, Bago R, Rajesh S, Overduin M, Berditchevski F, Florin L. The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses. Sci Rep. 6:32337.

Smithers CC, Overduin M. Structural Mechanisms and Drug Discovery Prospects of Rho GTPases. Cells. 5(2).

Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M. Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin. Nature Commun. 7:10827.

Stetefeld, J., McKenna, S. A. and Patel, T. R. Dynamic Light Scattering: A practical guide and applications in biomedical sciences. Biophys Rev 8, 409–427

Sarhan, A. R., Patel, T. R., Cowell, A. R., Tomlinson, M. G., Hellberg, C., Heath, J. K., Cunningham, D. L. and Hotchin, N. A. LAR protein tyrosine phosphatase regulates focal adhesions via CDK1. J Cell Science 129, 2962-2971

Sarhan, A. R., Patel, T. R., Creese, A. J., Tomlinson, M. G., Hellberg, C., Heath, J. K., Hotchin, N. A. and Cunningham, D. L. Regulation of platelet derived growth factor signaling by leukocyte common antigen-related (LAR) protein tyrosine phosphatase: A quantitative phosphoproteomics study. Mol Cell Proteomics 15, 1823-1836

Reuten, R., Nikodemus, D., Oliveria, M. B., Patel, T. R., Brachvogel, B., Breloy, I., Stetefeld, J. and Koch, M. Maltose-Binding Protein (MBP), a secretion-enhancing tag for mammalian protein expression systems. PLoS One 1(3):e0152386

Grandin, M., Meier, M., Delcrosa, J. G., Nikodemus, D., Reuten, R., Patel, T. R., Goldschneider, D., Orriss, G., Krahn, N., Boussouar, A., Abes, R., Dean, Y., Neves, D., Bernet, A., Depil, S., Schneiders, F., Poole, K., Dante, R., Koch, M., Mehlen, P. and Stetefeld, J. Structural decoding of the Netrin-1-UNC5 interaction and its therapeutical implications in cancers. Cancer Cell 29, 175-185

Patel, T. R., Nikodemus, D., Reuten, R., Besong, T. M., Meier, M., Winzor, D. J., Koch, M. and Stetefeld, J. Biophysical analysis of a lethal laminin alpha-1 mutation reveals altered self-interaction. Matrix Biology 49, 93-105

Patel, T. R., Winzor, D. J. and Scott, D. J. Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution. Methods 95, 55-61


Li MX, Hwang PM. Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs. Gene. 571:153-66.

Hwang PM, Sykes BD. Targeting the sarcomere to correct muscle function. Nat Rev Drug Discov. 14(5):313-28.

Lenoir M, Kufareva I, Abagyan R, Overduin M. Membrane and Protein Interactions of the Pleckstrin Homology Domain Superfamily. Membranes. 5:646-63.

Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M. G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Biosci Rep. 35 e00188.

Ariyo, E. O., Booy, E. P., Patel, T. R., Dzananovic, E., McRae, E. K., Meier, M., McEleney, K., Stetefeld, J. and McKenna, S. A. Biophysical characterization of G-quadruplex recognition in the PITX1 mRNA by the specificity domain of the helicase RHAU. PLoS ONE 10, e0144510.

Deo, S., Patel, T. R., Chojnowski, G., Koul, A., Dzananovic, E., McEleney, K., Bujnicki, J. M. and McKenna, S. A. Characterization of the termini of the West Nile virus genome and their interactions with the small isoform of the 2 5-oligoadenylate synthetase family. J Struct Biol 190, 236-249.

Vadlamani, G., Balcewich, M. D., Patel, T. R., Donald, L, J., Reeve, T. M., Stetefeld, J., Standing, K. G., Vocadlo, D. J. and Mark, B. L. The β – lactamase gene regulator AmpR is a tetramer that recognizes and binds the D-Ala-D-Ala motif of its repressor UDP-N acetylmuramic acid (MurNAc)-pentapeptide. J Biol Chem 290, 2630-2643.


Hwang PM, Cai F, Pineda-Sanabria SE, Corson DC, Sykes BD. The cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C. Proc Natl Acad Sci USA. 111:14412-7.

Koppisetti RK, Fulcher YG, Jurkevich A, Prior SH, Xu J, Lenoir M, Overduin M, Van Doren SR. Ambidextrous binding of cell and membrane bilayers by soluble matrix metalloproteinase-12. Nature Commun. 5:5552.

Kufareva I, Lenoir M, Dancea F, Sridhar P, Raush E, Bissig C, Gruenberg J, Abagyan R, Overduin M. Discovery of novel membrane binding structures and functions. Biochem Cell Biol. 92:555-63.

Butterworth S, Overduin M, Barr AJ. Targeting protein tyrosine phosphatase SHP2 for therapeutic intervention. Future Med Chem. 6:1423-37.

Lenoir M, Sugawara M, Kaur J, Ball LJ, Overduin M. Structural insights into the activation of the RhoA GTPase by the lymphoid blast crisis (Lbc) oncoprotein. J Biol Chem. 289:23992-4004.

NANUC entry

Chamber for NANUC's 800 MHz NMR spectrometer